Western blot examination of 0.5ug T7 Draw blend protein with Anti-T7 Tag monoclonal antibody in 1:1,000 (lane A), 1:2,000 (lane B), 1:5,000 (lane C) dilutions.Liquid in PBS, pH 7.4, comprising 0.02% sodium azide as preservative and 50% Glycerol.
Stable for twelve months at -20°C from day of shipment. For optimum healing of item, centrifuge the first vial following thawing and ahead of removing the cap. Aliquot in order to avoid recurring freezing and thawing.
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T7 tag is an epitope draw made up of an 11-residue peptide protected from the best choice sequence of the T7 bacteriophage gene10, which encodes a T7 important capsid protein. The T7 draw is generally manufactured onto the N- or C- terminus of a protein of curiosity so the branded protein can be analyzed and visualized using immunochemical methods.
The T7 tag has been applied carefully as a general epitope tag in several appearance vectors such as the pET process that is dependant on T7 RNA polymerase appearance systems.T7 draw; 10; Major capsid protein 10A; Key capsid protein; Small capsid protein 10B; T7 epitope.