Optimum working dilutions should really be decided experimentally by the investigator. Suggested starting dilutions are the following: WB 1:1000-2000.The antibody was affinity-purified from mouse ascites by affinity-chromatography using certain immunogen.
European blot analysis of TATA Binding Protein express in Hela (lane A), Mouse mind muscle (lane B) and Rat brain structure (lane C) with TBP/TATA Holding TBP antibody monoclonal antibody with 1:2000 dilution.
Fluid in PBS, pH 7.4, containing 0.02% salt azide as preservative and 50% Glycerol.Stable for 12 months at -20°C from time of shipment. For optimum healing of solution, centrifuge the original vial following thawing and just before removing the cap. Aliquot to avoid repeated cold and thawing.
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Normal transcription component that operates at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA package is the initial transcriptional step of the pre-initiation complex (PIC), playing a function in the initial of eukaryotic genes transcribed by RNA polymerase II.
Component of the transcription factor SL1/TIF-IB complicated, which can be mixed up in assembly of the PIC (preinitiation complex) all through RNA polymerase I-dependent transcription. The rate of PIC development probably is mainly determined by the charge of association of SL1 with the rDNA promoter. SL1 is involved with stabilization of nucleolar transcription element 1/UBTF on rDNA.
TBP; GTF2D1; TF2D; TFIID; TATA-box-binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box component; Transcription initiation factor TFIID TBP subunit.